LoFlo Medium Size:6 x 1kg These buffers were developed with

These buffers were developed with the following criteria in mind: midrange pKa

01 Suspend 22 gram powdered medium in 1 litre distilled water

C22H24N1O8·HCl = 480

Usage above a 20 mM concentration in mammalian cell culture work is not recommended

Friehs and Flaschel (2004)

LoFlo Medium Size:6 x 1kg These buffers were developed withFormula mg l Glucose 11000 g l KH2PO4 680. 45 g l Casein Peptone 5000 g l NH4Cl 26. 8 MgCl2 37. 1 CaCl2 1. 1 FeCl3 8. 11 Na2 EDTA 4. 84 ZnSO4 2. 30 H3BO3 1. 11 MnCl2. 4H2O 0. 51 CoCl2 0. 17 CuSO5. 5H2O 0. 15 (NH4)6M07O24. 4H2O 0. 1 Suspend 16. 8 gram powdered medium in 1 litre distilled water. Store dry at room temperature Adjust to pH 6. 5