SD Broth / 2% Glucose w/o Phosphate (KH2PO4) Size:500g Tryptone 10 Yeast extract 5

Tryptone 10 Yeast extract 5 NaCl 0

15 g SP supplements (Ade

at this strength the ph is around 6

Yeast extract 5 Dextrose 30 Total 35

32 KH2PO4 870 Carbon Source   Glucose 10000 Total 19334

SD Broth / 2% Glucose w/o Phosphate (KH2PO4) Size:500g Tryptone 10 Yeast extract 5SD Broth 2% Glucose w o Phosphate (KH2PO4) is based upon Yeast Nitrogen base without Amino acids and including glucose. The absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate, like P32 labeled components, or to select an optimal concentration of phosphate for certain yeast cell lines. Suspend 25. 9 gram powdered medium in 1 litre distilled water Store dry at room temperature